The Cell Science Core is supported by the Delaware INBRE, the Center for Pediatric Research, COBRE, and Nemours Biomedical Research to assist researchers at Nemours and affiliated institutions in cell science, proteomics and separation of proteins and small molecules. For more information, click on the list of facilities to the right. Please consult with the Director or staff for information on charges and procedures.
Cell Science Core Laboratory
Services
- 2D DIGE proteomics
- Fluorescence activated cell sorting (FACS)
- Flow cytometry (analysis)
- Whole blood analysis (non-clinical)
- HPLC of small molecules
- FPLC of proteins
- Fluorescence scanning
- Fluorescence plate reader
HEMA VET 950- 5 part WBC Hematology System has 24 reportable Parameters including platelets and full 5 part differential. As low as 20 micro litres of whole blood is enough for analysis. It provides superior cell separation for accurate and precise analysis.
Typhoon Trio™ Variable Mode Imager provides optimized detection of Cy2, Cy3, and Cy5 CyDye™ DIGE Fluor minimal and saturation dyes, enabling visualization of up to three differently labeled samples on a single gel.
VICTOR3™ is a multilabel, multitask plate reader for all light-emitting and light-absorbing technologies including: fluorescence, luminescence, absorbance, UV absorbance, time-resolved fluorometry,and fluorescence polarization.
Waters Alliance 2695 HPLC is a high performance liquid chromatographic system with a quaternary, low-pressure mixing pump and inline vacuum degassing. Flow rates from 50 uL/min to 5 mL/min can be generated for use with 2.1 mm ID columns and larger.The autosampler has a maximum capacity of 120 vials (12x32, 2-mL) with programmable temperature control from 4 to 40°C. The detector is a photodiode array with a wavelength range of 190-800 nm and sensitivity settings from 0.0001-2.0000 absorbance units.
Waters 650E Advanced Protein Purification System
Ettan IPGphor II: Proteins in complex mixtures are resolved on an immobilized pH gradient (IPG) gel according to their pI (1st dimension). A wide variety of IPG pH ranges, lengths, and loading techniques allows users to resolve whole proteomes for analysis and/or protein spot identification.
2D-DIGE: Protein samples are labeled with CyDye™ DIGE Fluors. Samples are then multi-plexed, using 2-Delectrophoresis, and analyzed using Decyder™ software. As little as 5 µg per sample can be used to analyze differences between proteomes.
Nemours Cell Science Core Laboratory Staff and Contact Information
Director: Robert W Mason, PhD
rmason@nemours.org
mason@nemoursresearch.org
(302) 651-6885
Laboratory Manager: Lisa Glazewski, MS
(302) 651-6892
INBRE coordinator: Aruna Sri Bojja, MS
(302) 651-6892
Websites: