Hemaprompt FT Occult Testing

1. Check expiration date on occult slide

2. Specimen processing

1. Use a thin smear from 2 different areas of the stool specimen or 2 drops of gastric fluid, apply to each window – leave an outer periphery
2. Close card & turn over
3. Grip where indicated. Lift up silver tab so the white developer pad is visible.
4. Pull silver table slowly all the way to the right and completely remove. Developer pad will “flip over as tab is pulled.
5. Wait 60 seconds before interpreting results.

3. Reading Results

1. Any blue color in the window after 60 seconds is considered a positive
2. Any other color is a negative result.

4. Internal Controls

1. Positive Control: Appearance of a Blue checkmark (√)
2. Negative Control: White background behind blue checkmark
   
   Note: Controls must appear for valid results.

5. Recording Results

1. Record the results on the appropriate form (POCT Daily Log If applicable) and on the patient flow sheet/EMR
2. Complete patient information and result on front of card (patient ID, date and sample source)
3. Complete Hemaprompt QC on front of card
4. Remove label and place on POCT logsheet along with patients 2^nd identifier, operator initials and time of test.

6. Interfering substances

1. Fecal Specimens
   1. Red and rare meats, horseradish, raw fruits and vegetables such as broccoli, cauliflower, red radish, cantaloupe, parsnips and turnips, or other high peroxidasecontaining vegetables, which can cause false positive results.
   2. Certain medications such as aspirin, indomethacin, phenylbutazone, reserpine, corticosteroids and non-steroidal anti-inflammatory drugs can cause gastrointestinal bleeding and therefore give positive results. Iron containing compounds may cause false positive results.
   3. Vitamin C in dosages greater than 250 mg per day has been shown to cause false negative results.
2. Gastric Specimens
   1. All foods and medications listed above may cause false positive (or negative) results in gastric specimens.
   2. Cimetidine (Tagamet) may cause false positive results