Dominique Darmaun, MD, PhD

Senior Investigator, Nemours Biomedical Research
Co-Director, Endocrine Research Laboratory

Department of Pediatrics
Division of Endocrinology

Education:

• Undergraduate: Bachelor of Science, University of Orleans, Orleans, France
• Medical School: University of Paris - St. Antoine Medical School, Paris, France
• Graduate: Masters in Human Physiology, University of Paris, Paris, France
• Post-Graduate: Doctorate in Human Nutrition, University of Paris, Paris, France

Post Graduate Training:

• Internship and Residency: Pediatrics, Hôpitaux de Paris
• Fellowship: Pediatric Endocrinology, Hôpitaux de Paris
• Fellowship: Research Fellow in Pediatrics, Metabolism Division, Washington University School of Medicine, St. Louis, Missouri

Certification:

• French Board of Pediatrics

Academic Appointment:

• Professor of Nutrition (University of Nantes, France)

Publications:

Tea I, Ferchaud-Roucher V, Küster A, Darmaun D, Robins RJ. Determination of ¹³C isotopic enrichment of glutathione and glycine by gas chromatography-combustion-isotope ratio mass spectrometry after formation of the N- or N,S-ethoxycarbonyl methyl ester derivatives. Rapid Commun. Mass Spec 2007, in press.

Humbert B, Nguyen P, Martin L, Dumon H, Vallette G, Maugère P, Darmaun D. Effect of glutamine on glutathione kinetics in vivo in dogs. J Nutr Biochem 2007; 18:10-16.

Le Bacquer O, Mauras N, Welch S, Haymond M, Darmaun D. Acute depletion of plasma glutamine increases leucine oxidation in prednisone-treated humans.Clinical Nutrition 2007; 26:231-238.

Han JC, Balagopal P, Sweeten S, Darmaun D, Mauras N. Evidence for hypermetabolism in boys with constitutional delay of growth and maturation. J Clin Endocrino Metab 2006; 91:2081-2086.

Pinel C, Coxam V, Mignon M, Taillandier D, Cubizolles C, Lebecque P, Darmaun D, Meynial-Denis D. Alterations in glutamine synthetase activity in rat skeletal muscle are associated with advanced age. Nutrition 2006; 22:778-785.

Balagopal P, Olney R, Darmaun D, Mougey E, Dokler M, Sieck G, Hammond D. Oxandrolone enhances skeletal muscle myosin synthesis and alters global gene expression profile in Duchenne muscular dystrophy. Am J Physiol Endocrinol Metab 2006; 290(3):E530-E539.

Darmaun D, Smith SD, Sweeten S, Sager BK, Welch S, Mauras N. Evidence for accelerated rates of glutathione utilization and glutathione depletion in adolescents with poorly controlled type 1 diabetes. Diabetes 2005; 54(1):190-196.

Darmaun D, Mauras N. Use of stable isotopes to assess protein and amino acid metabolism in children and adolescents: a brief review. Horm Res 2005; 64 Suppl 3:32-37.

Meynial-Denis D, Verdier L, Mignon M, Leclerc JN, Bayle G, Darmaun D. Does acute glutamine depletion enhance the response of glutamine synthesis to fasting in muscle in adult and old rats? Clin Nutr 2005; 24(3):398-406.

Bourreille A, Humbert B, Maugere P, Galmiche JP, Darmaun D. Glutamine metabolism in Crohns disease: a stable isotope study. Clin Nutr 2004; 23(5):1167-1175.

Darmaun D, Hayes V, Schaeffer D, Welch S, Mauras N. Effects of glutamine and recombinant human growth hormone on protein metabolism in prepubertal children with cystic fibrosis. J Clin Endocrinol Metab 2004; 89(3):1146-1152.

de la Cochetiere MF, Piloquet H, des RC, Darmaun D, Galmiche JP, Roze JC. Early intestinal bacterial colonization and necrotizing enterocolitis in premature infants: the putative role of Clostridium. Pediatr Res 2004; 56(3):366-370.

Rakotoambinina B, Marks L, Badran AM, Igliki F, Thuillier F, Crenn P, Messing B, Darmaun D. Taurine kinetics assessed using [1,2-13C2]taurine in healthy adult humans. Am J Physiol Endocrinol Metab 2004; 287(2):E255-E262.

Bocquet A, Bresson JL, Briend A, Chouraqui JP, Darmaun D, Dupont C, Frelut ML, Ghisolfi J, Goulet O, Putet G, Rieu D, Turck D, Vidailhet M, Merlin JP, Rives JJ. [Feeding of infants based on age. Practice guidelines]. Arch Pediatr 2003; 10(1):76-81.

Bocquet A, Bresson JL, Briend A, Chouraqui JP, Darmaun D, Dupont C, Frelut ML, Ghisolfi J, Girardet JP, Goulet O, Putet G, Rieu D, Rigo J, Turck D, Vidailhet M. [The morning snack at school is inadequate and unnecessary]. Arch Pediatr 2003; 10(11):945-947.

Le Bacquer O, Laboisse C, Darmaun D. Glutamine preserves protein synthesis and paracellular permeability in Caco-2 cells submitted to "luminal fasting". Am J Physiol Gastrointest Liver Physiol 2003; 285(1):G128-G136.

Rooyackers O, Darmaun D, Walrand S, Boirie Y, Bulow J, Ljungqvist O, Wernerman J. Report from the third meeting on tracer network for metabolic research. Clin Nutr 2003; 22(4):423.

Bocquet A, Bresson JL, Briend A, Chouraqui JP, Darmaun D, Dupont C, Frelut ML, Ghisolfi J, Goulet O, Putet G, Rieu D, Turck D, Vidailhet M. [Nutritional treatment of acute diarrhea in an infant and young child]. Arch Pediatr 2002; 9(6):610-619.

Bocquet A, Bresson JL, Briend A, Chouraqui JP, Darmaun D, Dupont C, Frelut ML, Ghisolfi J, Goulet O, Putet G, Rieu D, Turck D, Vidailhet M. [Fluoride supplementation must be initiated at birth in children in France]. Arch Pediatr 2002; 9(11):1211-1212.

Darmaun D. [Insulin, diabetes, and amino acid metabolism]. Journ Annu Diabetol Hotel Dieu 2002;105-115.

des RC, Le Bacquer O, Piloquet H, Roze JC, Darmaun D. Acute effects of intravenous glutamine supplementation on protein metabolism in very low birth weight infants: a stable isotope study. Pediatr Res 2002; 51(1):87-93.

Hayes V, Schaeffer D, Mauras N, Punati J, Darmaun D. Can glutamine and growth hormone promote protein anabolism in children with cystic fibrosis? Horm Res 2002; 58 Suppl 1:21-23.

Humbert B, Martin L, Dumon H, Darmaun D, Nguyen P. Dietary protein level affects protein metabolism during the postabsorptive state in dogs. J Nutr 2002; 132(6 Suppl 2):1676S-1678S.

Humbert B, Nguyen P, Dumon H, Deschamps JY, Darmaun D. Does enteral glutamine modulate whole-body leucine kinetics in hypercatabolic dogs in a fed state? Metabolism 2002; 51(5):628-635.

Ouguerram K, Krempf M, Maugeais C, Maugere P, Darmaun D, Magot T. A new labeling approach using stable isotopes to study in vivo plasma cholesterol metabolism in humans. Metabolism 2002; 51(1):5-11.

Pouteau EB, Mariot SM, Martin LJ, Dumon HJ, Mabon FJ, Krempf MA, Robins RJ, Darmaun DH, Naulet NA, Nguyen PG. Rate of carbon dioxide production and energy expenditure in fed and food-deprived adult dogs determined by indirect calorimetry and isotopic methods. Am J Vet Res 2002; 63(1):111-118.

Bocquet A, Bresson JL, Briend A, Chouraqui JP, Darmaun D, Dupont C, Frelut ML, Ghisolfi J, Goulet O, Putet G, Rieu D, Turck D, Vidailhet M. [Infant formulas and soy protein-based formulas: current data]. Arch Pediatr 2001; 8(11):1226-1233.

Humbert B, Le Bacquer O, Nguyen P, Dumon H, Darmaun D. Protein restriction and dexamethasone as a model of protein hypercatabolism in dogs: effect of glutamine on leucine turnover. Metabolism 2001; 50(3):293-298.

Humbert B, Bleis P, Martin L, Dumon H, Darmaun D, Nguyen P. Effects of dietary protein restriction and amino acids deficiency on protein metabolism in dogs. J Anim Physiol Anim Nutr (Berl) 2001; 85(7-8):255-262.

Humbert B, Nguyen P, Obled C, Bobin C, Vaslin A, Sweeten S, Darmaun D. Use of L-[(15)N] glutamic acid and homoglutathione to determine both glutathione synthesis and concentration by gas chromatography-mass spectrometry (GCMS). J Mass Spectrom 2001; 36(7):726-735.

Le Bacquer O, Nazih H, Blottiere H, Meynial-Denis D, Laboisse C, Darmaun D. Effects of glutamine deprivation on protein synthesis in a model of human enterocytes in culture. Am J Physiol Gastrointest Liver Physiol 2001; 281(6):G1340-G1347.

Crenn P, Thuillier F, Rakatoambinina B, Rongier M, Darmaun D, Messing B. Duodenal vs. gastric administration of labeled leucine for the study of splanchnic metabolism in humans. J Appl Physiol 2000; 89(2):573-580.

Darmaun D. Role of glutamine depletion in severe illness. Diabetes Nutr Metab 2000; 13(1):25-30.

Darmaun D, Humbert B. Does the fate of enterally administered glutamine depend on its molecular form? Bound versus free amino acid. Nutrition 2000; 16(11-12):1101-1102.

Hankard RG, Haymond MW, Darmaun D. Role of glucose in the regulation of glutamine metabolism in health and in type 1 insulin-dependent diabetes. Am J Physiol Endocrinol Metab 2000; 279(3):E608-E613.

Darmaun D. Role of nutrients in the regulation of in vivo protein metabolism in humans. Acta Paediatr Suppl 1999; 88(433):92-94.

Hankard R, Mauras N, Hammond D, Haymond M, Darmaun D. Is glutamine a conditionally essential amino acid in Duchenne muscular dystrophy? Clin Nutr 1999; 18(6):365-369.

Kuhn KS, Schuhmann K, Stehle P, Darmaun D, Furst P. Determination of glutamine in muscle protein facilitates accurate assessment of proteolysis and de novo synthesis-derived endogenous glutamine production. Am J Clin Nutr 1999; 70(4):484-489.

Liet JM, Piloquet H, Marchini JS, Maugere P, Bobin C, Roze JC, Darmaun D. Leucine metabolism in preterm infants receiving parenteral nutrition with medium-chain compared with long-chain triacylglycerol emulsions. Am J Clin Nutr 1999; 69(3):539-543.

Marchini JS, Nguyen P, Deschamps JY, Maugere P, Krempf M, Darmaun D. Effect of intravenous glutamine on duodenal mucosa protein synthesis in healthy growing dogs. Am J Physiol 1999; 276(4 Pt 1):E747-E753.

Darmaun D, Welch S, Rini A, Sager BK, Altomare A, Haymond MW. Phenylbutyrate-induced glutamine depletion in humans: effect on leucine metabolism. Am J Physiol 1998; 274(5 Pt 1):E801-E807.

Hankard RG, Hammond D, Haymond MW, Darmaun D. Oral glutamine slows down whole body protein breakdown in Duchenne muscular dystrophy. Pediatr Res 1998; 43(2):222-226.

Marchini JS, Marks LM, Darmaun D, Young VR, Krempf M. Hyperglucagonemia and the immediate fate of dietary leucine: a kinetic study in humans. Metabolism 1998; 47(5):497-502.

Messing B, Dutra SL, Thuillier F, Darmaun D, Desjeux JF. Whole-body protein metabolism assessed by leucine and glutamine kinetics in adult patients with active celiac disease. Metabolism 1998; 47(12):1429-1433.

Pouteau E, Maugere P, Darmaun D, Marchini JS, Piloquet H, Dumon H, Nguyen P, Krempf M. Role of glucose and glutamine synthesis in the differential recovery of 13CO2 from infused [2-13C] versus [1-13C] acetate. Metabolism 1998; 47(5):549-554.

Pouteau E, Dumon H, Nguyen P, Darmaun D, Champ M, Krempf M. Whole-body, peripheral and intestinal endogenous acetate turnover in dogs using stable isotopes. J Nutr 1998; 128(1):111-115.

Pouteau E, Vahedi K, Messing B, Flourie B, Nguyen P, Darmaun D, Krempf M. Production rate of acetate during colonic fermentation of lactulose: a stable-isotope study in humans. Am J Clin Nutr 1998; 68(6):1276-1283.

Carbonnel F, Messing B, Rimbert A, Rongier M, Koziet J, Darmaun D. Energy and protein metabolism during recovery from malnutrition due to nonneoplastic gastrointestinal disease. Am J Clin Nutr 1997; 65(5):1517-1523.

Darmaun D, Roig JC, Auestad N, Sager BK, Neu J. Glutamine metabolism in very low birth weight infants. Pediatr Res 1997; 41(3):391-396.

Hankard RG, Haymond MW, Darmaun D. Role of glutamine as a glucose precursor in fasting humans. Diabetes 1997; 46(10):1535-1541.

Maugeais C, Braschi S, Ouguerram K, Maugeais P, Mahot P, Jacotot B, Darmaun D, Magot T, Krempf M. Lipoprotein kinetics in patients with analbuminemia. Evidence for the role of serum albumin in controlling lipoprotein metabolism. Arterioscler Thromb Vasc Biol 1997; 17(7):1369-1375.

Menand C, Pouteau E, Marchini S, Maugere P, Krempf M, Darmaun D. Determination of low 13C-glutamine enrichments using gas chromatography-combustion-isotope ratio mass spectrometry. J Mass Spectrom 1997; 32(10):1094-1099.

Roze JC, Darmaun D, Gaultier C. Oxygen consumption in infants with bronchopulmonary dysplasia. Pediatr Pulmonol Suppl 1997; 16:172-173.

Hankard RG, Haymond MW, Darmaun D. Effect of glutamine on leucine metabolism in humans. Am J Physiol 1996; 271(4 Pt 1):E748-E754.

Salman EK, Haymond MW, Bayne E, Sager BK, Wiisanen A, Pitel P, Darmaun D. Protein and energy metabolism in prepubertal children with sickle cell anemia. Pediatr Res 1996; 40(1):34-40.

van Loon FP, Banik AK, Nath SK, Patra FC, Wahed MA, Darmaun D, Desjeux JF, Mahalanabis D. The effect of L-glutamine on salt and water absorption: a jejunal perfusion study in cholera in humans. Eur J Gastroenterol Hepatol 1996; 8(5):443-448.

Bright GM, Darmaun D. Corticosteroid-binding globulin modulates cortisol concentration responses to a given production rate. J Clin Endocrinol Metab 1995; 80(3):764-769.

Carbonnel F, Messing B, Darmaun D, Rimbert A, Rongier M, Rigal O, Koziet J, Thuillier F, Desjeux JF. Energy and protein metabolism in malnutrition due to nonneoplastic gastrointestinal diseases. Metabolism 1995; 44(9):1110-1115.

Hankard RG, Darmaun D, Sager BK, DAmore D, Parsons WR, Haymond M. Response of glutamine metabolism to exogenous glutamine in humans. Am J Physiol 1995; 269(4 Pt 1):E663-E670.

Spear ML, Darmaun D, Sager BK, Parsons WR, Haymond MW. Use of [13C]bicarbonate infusion for measurement of CO2 production. Am J Physiol 1995; 268(6 Pt 1):E1123-E1127.

Darmaun D, Just B, Messing B, Rongier M, Thuillier F, Koziet J, Grasset E. Glutamine metabolism in healthy adult men: response to enteral and intravenous feeding. Am J Clin Nutr 1994; 59(6):1395-1402.

Hankard R, Goulet O, Ricour C, Rongier M, Colomb V, Darmaun D. Glutamine metabolism in children with short-bowel syndrome: a stable isotope study. Pediatr Res 1994; 36(2):202-206.

Mauras N, Haymond MW, Darmaun D, Vieira NE, Abrams SA, Yergey AL. Calcium and protein kinetics in prepubertal boys. Positive effects of testosterone. J Clin Invest 1994; 93(3):1014-1019.

Bright GM, Parsons WR, Darmaun D, Rodrigues A, Haymond MW. Gas chromatographic-mass spectrometric method for measuring stable isotope enrichments of underivatized cortisol from small plasma volumes. J Chromatogr 1993; 621(1):1-6.

Darmaun D, DAmore D, Haymond MW. Determination of glutamine and alpha-ketoglutarate concentration and specific activity in plasma using high-performance liquid chromatography. J Chromatogr 1993; 620(1):33-38.

Goulet O, DePotter S, Salas J, Robert JJ, Rongier M, Ben Hariz M, Koziet J, Desjeux JF, Ricour C, Darmaun D. Leucine metabolism at graded amino acid intakes in children receiving parenteral nutrition. Am J Physiol 1993; 265(4 Pt 1):E540-E546.

Robert JJ, Chauvet D, Darmaun D, Leblanc H. Hepatic glucose production during intraperitoneal and intravenous closed-loop insulin regulation of blood glucose in type 1 (insulin-dependent) diabetic patients. Diabetologia 1993; 36(11):1185-1190.

Robert JJ, Rakotoambinina B, Cochet I, Foussier V, Magre J, Darmaun D, Chevenne D, Capeau J. The development of hyperglycaemia in patients with insulin-resistant generalized lipoatrophic syndromes. Diabetologia 1993; 36(12):1288-1292.

Darmaun D. [In vivo exploration of glutamine metabolism in man]. Diabete Metab 1992; 18(1 Pt 2):117-121.

Nath SK, Dechelotte P, Darmaun D, Gotteland M, Rongier M, Desjeux JF. [15N]- and [14C]glutamine fluxes across rabbit ileum in experimental bacterial diarrhea. Am J Physiol 1992; 262(2 Pt 1):G312-G318.

Darmaun D, Messing B, Just B, Rongier M, Desjeux JF. Glutamine metabolism after small intestinal resection in humans. Metabolism 1991; 40(1):42-44.

Darmaun D, Rongier M, Koziet J, Robert JJ. Glutamine nitrogen kinetics in insulin-dependent diabetic humans. Am J Physiol 1991; 261(6 Pt 1):E713-E718.

Darmaun D, Dechelotte P. Role of leucine as a precursor of glutamine alpha-amino nitrogen in vivo in humans. Am J Physiol 1991; 260(2 Pt 1):E326-E329.

Dechelotte P, Darmaun D, Rongier M, Hecketsweiler B, Rigal O, Desjeux JF. Absorption and metabolic effects of enterally administered glutamine in humans. Am J Physiol 1991; 260(5 Pt 1):G677-G682.

Just B, Messing B, Darmaun D. Oral nutrition in patients receiving home cyclic parenteral nutrition: pattern of substrate utilization. Am J Clin Nutr 1991; 54(3):560-564.

Just B, Darmaun D, Koziet J, Rongier M, Messing B. Use of a ventilated canopy for assessment of [13C]leucine oxidation in patients receiving total parenteral nutrition. JPEN J Parenter Enteral Nutr 1991; 15(1):65-70.

Just B, Messing B, Darmaun D, Rongier M, Camillo E. Comparison of substrate utilization by indirect calorimetry during cyclic and continuous total parenteral nutrition. Am J Clin Nutr 1990; 51(1):107-111.

Robert JJ, Magre J, Reynet C, Darmaun D, Picard J, Capeau J. In vivo and in vitro characterization of insulin resistance in three cases of lipoatrophic diabetes. Diabete Metab 1990; 16(3):240-247.

Darmaun D, Froguel P, Rongier M, Robert JJ. Amino acid exchange between plasma and erythrocytes in vivo in humans. J Appl Physiol 1989; 67(6):2383-2388.

Dechelotte P, Darmaun D, Rongier M, Desjeux JF. Glutamine transport in isolated rabbit ileal epithelium. Gastroenterol Clin Biol 1989; 13(10):816-821.

Slama G, Boillot J, Hellal I, Darmaun D, Rizkalla SW, Orvoen-Frija E, Dore MF, Guille G, Fretault J, Coursaget J. Fructose is as good a fuel as glucose for exercise in normal subjects. Diabete Metab 1989; 15(3):105-106.

Darmaun D, Matthews DE, Bier DM. Physiological hypercortisolemia increases proteolysis, glutamine, and alanine production. Am J Physiol 1988; 255(3 Pt 1):E366-E373.

Darmaun D, Cirillo D, Koziet J, Chauvet D, Young VR, Robert JJ. Whole body glucose kinetics in type I diabetes studied with [6,6-2H] and [U-13C]-glucose and the artificial B-cell. Metabolism 1988; 37(5):491-498.

Darmaun D, Matthews DE, Desjeux JF, Bier DM. Glutamine and glutamate nitrogen exchangeable pools in cultured fibroblasts: a stable isotope study. J Cell Physiol 1988; 134(1):143-148.

Robert JJ, Koziet J, Chauvet D, Darmaun D, Desjeux JF, Young VR. Use of 13C-labeled glucose for estimating glucose oxidation: some design considerations. J Appl Physiol 1987; 63(5):1725-1732.

Darmaun D, Robert JJ, Matthews DE, Young VR, Bier DM. [Regulation of protein metabolism by cortisol and insulin. In vivo study in man using amino acids labeled with stable isotopes]. Journ Annu Diabetol Hotel Dieu 1986;55-63.

Darmaun D, Robert JJ, Chevrot M, Dieterlen P, Reach G, Desjeux JF. [Glomerular filtration and tubular reabsorption of glucose in insulin-dependent diabetes in children]. Arch Fr Pediatr 1986; 43(1):23-27.

Darmaun D, Matthews DE, Bier DM. Glutamine and glutamate kinetics in humans. Am J Physiol 1986; 251(1 Pt 1):E117-E126.

Robert JJ, Darmaun D, Reach G, Lestradet H. [Blood and urinary glucose for optimal control of diabetes in children]. Ann Pediatr (Paris) 1986; 33(10):901-906.

Robert JJ, Darmaun D, Reach G, Lestradet H. [Artificial pancreas in diabetic children]. Presse Med 1986; 15(43):2159-2162.

Darmaun D, Manary MJ, Matthews DE. A method for measuring both glutamine and glutamate levels and stable isotopic enrichments. Anal Biochem 1985; 147(1):92-102.