Biomolecular Core Laboratory

The Biomolecular Core Laboratory (BCL) provides essential services in molecular biology and genetics to the clinical and research staff at Nemours, INBRE partner institutions, and external clients. BCL is under the direction of Dr. Katia Sol-Church and staffed by Jennifer Holbrook, Deborah Stabley, and Ellen Hitchens. The BCL provides consultation on experimental design and data troubleshooting, training, and mentoring of students and clinical fellows.

Molecular Services include:

Expression Analysis:

Affymetrix Microarray (global gene expression 3’IVT & WT assays)
Pathway-focused Real-Time qPCR (mRNA and miRNA)

Genotyping:

Allelic Discrimination Probes (SNP Real-Time PCR)
Affymetrix Microarray (CNV Cyto 2.7M, SNP arrays)
Fragment Analysis (Capillary Electrophoresis up to 1200 bp)
DNA Sequencing (Pyrosequencing and Sanger Sequencing)
High Resolution Melting Analysis (Real-Time PCR)

Methylation Analysis:

Global and Gene-Specific (pyrosequencing)
Bisulfite Sanger Sequencing and Pyrosequencing

Shared Instrumentation:

Agilent Bioanalyzer Lab-On-A-Chip, Beckman Biomek 2000 Liquid Handler, NanoDrop ND1000, Stratagene Gradient Robocycler, ABI7900 384-well Real-Time Genetic Analyzer, Biotage PSQ96 MA, Affymetrix GeneChip System 3000, PCR Tamer, Thermocyclers

CLIA-Certified Site: Operating under strict HIPAA regulations, the service core unit currently supports the Nemours Molecular Diagnostic DNA sequencing and fragment analysis CLIA-certified tests.

Delaware Valley Network: The Nemours Biomolecular Core Laboratory provides backup services to other cores in the Delaware Valley Network including DNA sequencing coverage services for the University of Delaware DNA Sequencing and Genotyping Core.

Upcoming Events

Northeast Regional Life Sciences Core Directors Meeting
October 27-29, 2010
University of Massachusetts
Worcester, MA

The American Society of Human Genetics
60th Annual Meeting
November 2-6, 2010
Washington, DC

The National Center for Research Resources (NCRR) at the National Institutes of Health (NIH) presents:
Moving Forward in the Efficient Management and Use of Core Facilities
November 15 – 16, 2010
Natcher Conference Center (Bldg. 45)
National Institutes of Health
45 Center Drive
Bethesda, MD 20892