The Histotechnology Core Lab in Nemours Biomedical Research is a full-service histology laboratory located on the campus of the Alfred I. duPont Hospital for Children in Wilmington, DE. In continuous operation since 1986, the Core Lab is CLIA-certified with two Histology Research Specialists and two Clinical Certified Specialists, HT (ASCP). The Lab has produced many posters and publications and also offers histology advisement for research project development. The Core serves both the research and clinical communities throughout Nemours.
Histotechnology Core Laboratory (HCL)

Services
- Routine histology and special staining
- Histochemistry and immuno-histochemistry
- In situ hybridization/in situ PCR
- Imaging and muscle histograms
- Laser microdissection
- Cytology, cytospins and cell membrane staining
- Antibody and probe development
- Frozen, paraffin and plastic techniques
- Histology advisement for research projects
Equipment
(1) Leica processor
(2) Leica cryostats
(2) Leica automated microtomes
(1) Sukura H&E stainer
(1) LabVision immuno-stainer
(1) Leica embedding center
(2) Olympus Light microsopes
(1) Olympus Fluorescence microscope
(1) Leica laser microdissector with FL scope
(1) HiTech imaging system with ImagePro Plus software
Contact Information
Carol A Barone, HT (ASCP) BAAS
Director
302-651-6827 Office
302-651-6771 Laboratory
302-651-5010 FAX
Staff
Bobbie Boyce, Histology Specialist III
Terry Kokas, Histology Specialist I
Brian Owens, HT (ASCP), Research Assistant I
