About Histochemistry and Tissue Processing Core Lab
The Histochemistry and Tissue Processing Core is a full service histology laboratory located on the campus of Nemours/Alfred I. duPont Hospital for Children. The Core has been in continuous operation since 1986, providing numerous routine and complex histology services from H&E (Hematoxylin Eosin Y) staining to complex molecular histology techniques.
The HTPC is CLIA certified and provides the highest quality outcomes using the latest instrumentation required of a full service histology lab.
Technology for routine and specialized research:
H&E and Special Staining
Muscle Enzyme Histochemistry
Immunohistochemistry (Fluorescent and IHC)
In Situ Hybridization, In Situ PCR and In Situ RT-PCR
Laser Capture, Micro-dissection (LCM/LMD)
The Core is staffed by ASCP certified technicians, technologist and research specialist with over 40 years of experience in the field of Histotechnology.
The Core can provide consultation for research projects, development of new antibodies for IHC, or probes for In situ Hybridization. Our Tissue Microarray lab can prepare slides from normal controls or provide custom arrays from provided sample blocks.
The Tissue Processing Core serves internal, external and corporate users, with excellent turn- around-time and competitive pricing. The Core has authored numerous abstracts, posters and publications, and has received awards in photomicrography and LEAN management.
Current Research Group
Brian Owens, Clinical Research Assistant II, HT (ASCP)
Terry Kokas, Histology Specialist III
Bobbie Boyce, Histology Specialist III (Lead Tech)
Heather Hardy, Assistant Director, HTL (ASCP)
Carol Barone, HT (ASCP) BAAS, C-IHC, C-Mol. Histo – Core Technical Director
Services & Equipment
We are unique, diverse, skilled, and user-friendly, and offer truly a complete histology service for both clinical and research investigators. With a qualified staff and a wide array of instrumentation for a variety of histology services, the lab has an excellent turnaround time for special staining, enzyme histochemistry, immuno-histochemistry and routine processing.
Routine histology and special staining
Histochemistry and immuno-histochemistry
In situ hybridization/in situ PCR
Imaging and muscle histograms
Cytology, cytospins, and cell membrane staining
Antibody and probe development
Frozen, paraffin, and plastic techniques
Histology advisement for research projects
(number of each)
(1) Leica processor
(2) Leica cryostats
(2) Leica automated microtomes
(1) Sukura H&E stainer
(1) LabVision immuno-stainer
(1) Leica embedding center
(2) Olympus Light microsopes
(1) Olympus Fluorescence microscope
(1) Leica laser microdissector with FL scope
(1) HiTech imaging system with ImagePro Plus software