The Histotechnology Core Lab (HCL) is a full-service histology (microscopic study of cells and tissues) lab located on the campus of the Nemours/Alfred I. duPont Hospital for Children in Wilmington, DE.
In continuous operation since 1986, the Core Lab has been certified by the Clinical Laboratory Improvement Amendments (CLIA — an effort by the U.S. Centers for Medicare & Medicaid Services to ensure quality lab testing) since 1996 with no deficiencies.
Serving external investigators and Nemours’ own research and clinical communities, the lab also offers histology advisement for research project development. With two Histology Research Specialists and two Clinical Certified Specialists, HT (ASCP), we have produced many posters and publications, as well as received awards in micro-photography and LEAN management, among others.
Current Research Group
Carol A. Barone, HT (ASCP) BAAS, Director
Bobbie Boyce, Histology Specialist III
Terry Kokas, Histology Specialist I
Brian Owens, HT (ASCP), Research Assistant I
Services & Equipment
We are unique, diverse, skilled, and user-friendly, and offer truly a complete histology service for both clinical and research investigators. With a qualified staff and a wide array of instrumentation for a variety of histology services, the lab has an excellent turnaround time for special staining, enzyme histochemistry, immuno-histochemistry, and routine processing.
Routine histology and special staining
Histochemistry and immuno-histochemistry
In situ hybridization/in situ PCR
Imaging and muscle histograms
Cytology, cytospins, and cell membrane staining
Antibody and probe development
Frozen, paraffin, and plastic techniques
Histology advisement for research projects
(number of each)
(1) Leica processor
(2) Leica cryostats
(2) Leica automated microtomes
(1) Sukura H&E stainer
(1) LabVision immuno-stainer
(1) Leica embedding center
(2) Olympus Light microsopes
(1) Olympus Fluorescence microscope
(1) Leica laser microdissector with FL scope
(1) HiTech imaging system with ImagePro Plus software