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Nemours Biomedical Research

(302) 651-6884


Histochemistry and Tissue Processing Core Lab

Nemours Biomedical Research

About Histochemistry and Tissue Processing Core Lab

The Histochemistry and Tissue Processing Core is a full service histology laboratory located on the campus of Nemours/Alfred I. duPont Hospital for Children. The Core has been in continuous operation since 1986, providing numerous routine and complex histology services from H&E (Hematoxylin Eosin Y) staining to complex molecular histology techniques.

The HTPC is CLIA certified and provides the highest quality outcomes using the latest instrumentation required of a full service histology lab.

Technology for routine and specialized research:
  • H&E and Special Staining
  • Muscle Enzyme Histochemistry
  • Immunohistochemistry (Fluorescent and IHC)
  • In Situ Hybridization, In Situ PCR and In Situ RT-PCR
  • Laser Capture, Micro-dissection (LCM/LMD)
  • Tissue photomicrography

The Core is staffed by ASCP certified technicians, technologist and research specialist with over 40 years of experience in the field of Histotechnology.

The Core can provide consultation for research projects, development of new antibodies for IHC, or probes for In situ Hybridization. Our Tissue Microarray lab can prepare slides from normal controls or provide custom arrays from provided sample blocks.

The Tissue Processing Core serves internal, external and corporate users, with excellent turn- around-time and competitive pricing. The Core has authored numerous abstracts, posters and publications, and has received awards in photomicrography and LEAN management.

Current Research Group
  • Brian Owens, Clinical Research Assistant II, HT (ASCP)
  • Terry Kokas, Histology Specialist III
  • Bobbie Boyce, Histology Specialist III (Lead Tech)
  • Heather Hardy, Assistant Director, HTL (ASCP)
  • Carol Barone, HT (ASCP) BAAS, C-IHC, C-Mol. Histo – Core Technical Director

Services & Equipment

We are unique, diverse, skilled, and user-friendly, and offer truly a complete histology service for both clinical and research investigators. With a qualified staff and a wide array of instrumentation for a variety of histology services, the lab has an excellent turnaround time for special staining, enzyme histochemistry, immuno-histochemistry and routine processing.

Services
  • Routine histology and special staining
  • Histochemistry and immuno-histochemistry
  • In situ hybridization/in situ PCR
  • Imaging and muscle histograms
  • Laser microdissection
  • Cytology, cytospins, and cell membrane staining
  • Antibody and probe development
  • Frozen, paraffin, and plastic techniques
  • Histology advisement for research projects
  • Photo-microscopy/imaging
Equipment

(number of each)

  • (1) Leica processor
  • (2) Leica cryostats
  • (2) Leica automated microtomes
  • (1) Sukura H&E stainer
  • (1) LabVision immuno-stainer
  • (1) Leica embedding center
  • (2) Olympus Light microsopes
  • (1) Olympus Fluorescence microscope
  • (1) Leica laser microdissector with FL scope
  • (1) HiTech imaging system with ImagePro Plus software

Contact Histotechnology Core Laboratory

Contact Information

Phone: Office: (302) 651-6827 | Lab: (302) 651-6771
Fax: (302) 651-5010
carol.barone@nemours.org

Lab hours

7:30 a.m. to 4:30 p.m. (Monday–Friday)