About Histochemistry and Tissue Processing Core Lab
The Histochemistry and Tissue Processing Core, (HTPC) is a full service histology laboratory providing routine and specialty histology techniques for internal, external academic and corporate investigators. As part of the Department of Biomedical Research at the Nemours/Alfred I. duPont Hospital for Children, the Core has been in continuous operation since 1986 and is part of the Delaware’s IDeA Network of Biomedical Research Excellence (INBRE). The HTPC has published numerous papers, posters, abstracts and articles that have contributed to the works of many investigators. The lab follows LEAN practices, is CLIA certified, and is staffed by ASCP licensed histologists and research technicians.
The HTPC has a wide array of instrumentation, and provides consultation for research projects for special staining, enzyme histochemistry, immuno-histochemistry and routine processing. The lab offers TMA services, and can prepare slides from normal controls or provide custom arrays from provided sample blocks.
Technology for routine and specialized research:H&E and Special Staining
Muscle Enzyme Histochemistry
Immunohistochemistry (Fluorescent and IP)
In Situ Hybridization, In Situ PCR and In Situ RT-PCR
Tissue Microarrays (TMA)
Current Research Group
Brian Owens, Clinical Research Assistant I
Terry Kokas, Histology Specialist III
Bobbie Boyce, Histology Specialist III (Lead Tech)
Heather Hardy, Histochemistry and Tissue Processing Core Director
The HTPC is unique, diverse, skilled, user-friendly and offers complete histology services. Go to the tab on the upper right to get our HTPC work request form.
Services & Equipment
The HTPC has a wide array of instrumentation for a variety of histology services, and offers excellent turnaround time for special staining, enzyme histochemistry, immuno-histochemistry and routine processing.
Routine histology and special staining
Histochemistry and immuno-histochemistry
In situ hybridization/in situ PCR
Imaging and muscle histograms
Cytology, cytospins, and cell membrane staining
Antibody and probe development
Frozen, paraffin, and plastic techniques
Histology consultation for research projects
(number of each)
(1) Leica processor
(2) Leica cryostats
(3) Leica automated microtomes
(1) Sakura H&E stainer
(1) Leica immuno-stainer
(1) Leica embedding center
(1) Thermo Scientific HistoStar embedding center TM.
(2) Olympus light microsopes
(1) Olympus fluorescence microscope
(1) HiTech imaging system with ImagePro Plus software