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Nemours Biomedical Research

(302) 651-6884


Gastroenterology Clinical Lab

Nemours Biomedical Research

Clinical Lab Services

Located at Nemours/Alfred I. duPont Hospital for Children in Wilmington, Delaware, the CLIA-certified GI Clinical Lab is a pediatric lab that provides a variety of commonly used clinical lab services. We also accept samples for research studies and clinical trials.

 
Disaccharidase Determination
Small Bowel Biopsy

The activity of disaccharidases, including lactase, maltase, sucrase, palatinase and glucoamylase, can be determined from an intestinal biopsy with approximately 2 to 5 mg wet weight.

Sample Preparation:
  • Avoid contamination with traces of the fixative used for surgical pathology biopsies.
  • Upon collection, the specimen should be placed in a small, tightly capped, plastic tube and immediately frozen on dry ice or stored in a freezer at -20°C to -70°C (no automatic defrost cycles).
  • The biopsy should not be placed on gauze or a toothpick, nor should any solutions be added.
  • The sample should be shipped with enough dry ice to remain frozen. Enzyme activity may be affected if the sample thaws.
 
Pancreatic Enzyme Activities
Duodenal Aspirate

Amylase, lipase, trypsin, chymotrypsin and elastase activities can be determined from duodenal fluid.

Sample Preparation:
  • A minimum of 0.2 ml fluid is required to analyze the five enzymes, protein content, and pH.
  • The sample can be a single fluid or multiple fluids.
  • The fluid should be placed in a small, tightly capped, plastic tube.
  • Keep the sample frozen and ship on dry ice.
 
Gastric Pepsin Assay

Gastric pepsin detected in the airway of patients is a specific and sensitive marker to assess pulmonary aspiration. The GI Clinical Lab at duPont Hospital for Children offers a sensitive, reliable enzymatic test to detect gastric pepsin in the airway samples. The assay has high specificity as it detects pepsin A that is exclusively expressed in the gastric mucosa. For more information on the research studies that used the assay or a similar enzymatic assay, please see the References listed below.

Sample Preparation:
  • Collecting airway aspirate: To collect tracheal secretion: Attach a LUKI trap to a suction catheter, pass the catheter down the endotracheal tube, and apply suction to the catheter as the catheter is withdrawn. If the secretion is too thick to be withdrawn, instill saline (2 cc) to the tracheal tube prior to aspirating the secretion.
  • Collecting bronchial secretion: Bronchial secretion can be collected using a standard collection protocol. Collect 1-2 cc bronchial washing fluid in a plain specimen tube.
  • Please avoid over-diluting the secretion with a large amount of saline during the collection.
  • A minimum of 0.5 cc fluid is required to determine gastric pepsin activity, protein content, and pH.
  • The airway fluid should be transferred to a small, tightly capped, plastic tube and frozen immediately after collection.
  • Keep sample frozen and ship on dry ice.

Clinical Labs for Your Child

The GI Clinical Lab at duPont Hospital for Children also accepts samples for research studies and clinical trials. As part of the Gastroenterology Lab, we provide services to develop research projects for industry and research institutions.

Current research collaborators include Mayo Clinic, Florida; Cooper University Hospital, New Jersey; and Digestive Care, Inc., Pennsylvania.

Learn More About Clinical Trials at Nemours »

Contact Gastroenterology Clinical Lab

Zhaoping He, PhD

Research Scientist
(302) 651-6853
zhaoping.he@nemours.org

Location:

Nemours/Alfred I. duPont Hospital for Children
1600 Rockland Road
Wilmington, DE 19803

Clinical Diagnostics

Our Center for Clinical Diagnostics provides diagnostic testing services to support patient care in a variety of medical specialties.
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