The Cell Science Core (CSC) assists researchers at Nemours and affiliated institutions in cell science, proteomics and separation of proteins and small molecules. The CSC is supported by the Delaware Institutional Development Award/IDeA Networks of Biomedical Research Excellence (INBRE), the Center for Pediatric Research, and the Center for Pediatric Research (COBRE).
The CSC is a component of the Clinical Biochemistry Lab, and supports the Nemours Biobank in preparing human samples suitable for protein, RNA, DNA, or whole-cell analysis. The lab provides access to state-of-the-art technology to investigators both in-house and at associated institutions within our INBRE/COBRE network.
Our staff works closely with investigators to design, perform, and analyze experiments. We also provide access to associated cores at neighboring institutions for instrumentation not available on site. Additional support is available for project development. A fee-for-service process has been established. Mini-grants and core fee waivers may be available. Please consult with the director or staff for information on charges and procedures.
This system has 24 reportable parameters, including platelets and full 5-part differential. As low as 20 micro litres of whole blood is enough for analysis. It provides superior cell separation for accurate and precise analysis.
Typhoon Trio™ Variable Mode Imager
Typhoon Trio™ Variable Mode Imager provides optimized detection of Cy2, Cy3, and Cy5 CyDye™ DIGE Fluor minimal and saturation dyes, enabling visualization of up to three differently labeled samples on a single gel.
VICTOR3™ is a multilabel, multitask plate reader for all light-emitting and light-absorbing technologies including: fluorescence, luminescence, absorbance, UV absorbance, time-resolved fluorometry, and fluorescence polarization.
Waters Alliance 2695 HPLC
Waters Alliance 2695 HPLC is a high performance liquid chromatographic system with a quaternary, low-pressure mixing pump and inline vacuum degassing. Flow rates from 50 uL/min to 5 mL/min can be generated for use with 2.1 mm ID columns and larger. The autosampler has a maximum capacity of 120 vials (12x32, 2-mL), with programmable temperature control from 4 to 40°C. The detector is a photodiode array with a wavelength range of 190-800 nm and sensitivity settings from 0.0001-2.0000 absorbance units.
Waters 650E Advanced Protein Purification System
Ettan IPGphor II
Proteins in complex mixtures are resolved on an immobilized pH gradient (IPG) gel according to their pI (1st dimension). A wide variety of IPG pH ranges, lengths, and loading techniques allows users to resolve whole proteomes for analysis and/or protein spot identification.
Protein samples are labeled with CyDye™ DIGE Fluors. Samples are then multi-plexed, using 2-Delectrophoresis, and analyzed using Decyder™ software. As little as 5 µg per sample can be used to analyze differences between proteomes.